Salting-out-assisted liquid–liquid extraction as a suitable approach for determination of methoxetamine in large sets of tissue samples
Analytical and Bioanalytical Chemistry December 11, 2015 Kateřina Hájková, Bronislav Jurásek, David Sýkora et al. 26 citations
A new sample preparation and detection method for the designer drug methoxetamine (MXE) and five of its metabolites in rat brain, liver, and lung tissues was developed. The procedure uses salting-out-assisted liquid-liquid extraction followed by liquid chromatography-tandem mass spectrometry. Calibration curves were linear from 2.5 to 250 ng/g, with limits of quantification of 2.5 and 5 ng/g. Recovery ranged from 80% to 117% and matrix effects from 94% to 110%. The method was successfully applied to real samples from toxicological trials on rats.