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A rapid colorimetric assay (PSILO QTest) was deployed during a 7-day psilocybin retreat in Jamaica to measure batch-level psilocybin concentration in Psilocybe cubensis mushrooms. Psilocybin content differed approximately 2.6-fold between two session batches: one batch averaged 6.095 mg/g, the other 15.75 mg/g. Estimated psilocybin-equivalent doses ranged from 15.2 to 21.3 mg in Session 1 and 47.1 to 70.7 mg in Session 2, showing that gram-based dosing poorly reflects actual psychoactive substance exposure. No serious adverse events were observed among 11 participants. The assay can be used by non-chemist facilitators to improve dose documentation in naturalistic psilocybin research.