[3H]1-[2-(2-thienyl)cyclohexyl]piperidine labels two high-affinity binding sites in human cortex: further evidence for phencyclidine binding sites associated with the biogenic amine reuptake complex.
Synapse (New York, N.Y.) August 1, 1991 H C Akunne, A A Reid, A Thurkauf et al.
The brain contains two types of PCP binding sites: site 1 (linked to the NMDA receptor) and site 2 (linked to the dopamine reuptake complex). This work examined brain membranes from multiple species and found detectable site 2 in guinea pig, rabbit, pig, mouse, sheep, and human, but not in rat or chicken. In human cortex, site 2 was the predominant binding site. Dopamine and serotonin uptake inhibitors produced a wash-resistant inhibition of [3H]TCP binding to site 2 but not site 1. The results suggest human cortex possesses high-affinity PCP binding sites associated with biogenic reuptake binding sites, and that guinea pig, but not rat, may be an appropriate animal model for studying PCP site 2 in human brain.