Application of a nucleophilic substitution rection for spectrofluorimetric quantification of ketamine in pharmaceutical and plasma matrices.
Scientific reports December 30, 2024 Reem M Alnemari, Maram H Abduljabbar, Yusuf S Althobaiti et al.
A new, environmentally friendly spectrofluorimetric method detects ketamine in pharmaceutical and plasma samples with high sensitivity. The method relies on a nucleophilic substitution reaction between ketamine and NBD-Cl, producing a fluorescent derivative measurable at 543 nm emission after 481 nm excitation. Over a concentration range of 10–250 ng/mL, fluorescence intensity correlates linearly with ketamine concentration. Detection and quantitation limits are 2.37 ng/mL and 7.18 ng/mL, respectively. The method is precise (relative standard deviation < 1), accurate (mean recovery 99.74%), and robust (recovery 98.00–99.97%). Compared to existing methods, it is more sensitive and ecologically benign.