Optimization of enantioselective high-performance liquid chromatography-tandem mass spectrometry method for the quantitative determination of 3,4-methylenedioxy-methamphetamine (MDMA) and its phase-1 metabolites in human biological fluids.
Journal of pharmaceutical and biomedical analysis June 15, 2024 Giorgia Sprega, Giorgi Kobidze, Alfredo Fabrizio Lo Faro et al. 4 citations
An improved chiral LC-MS/MS method separates all four pairs of enantiomers of MDMA and its major phase-1 metabolites (HMA, HMMA, MDA) on a single Lux AMP column within six minutes, using an optimized mobile phase and column dimensions. The method was applied to human plasma, oral fluid, and urine. In urine, hydrolysis of glucuronides with hydrochloric acid or glucuronidase was tested to evaluate effects on the concentration and enantiomeric distribution of the hydroxy metabolites HMA and HMMA.