Bezhan Chankvetadze

Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, I. Chavchavadze Ave 1, Tbilisi 0179, Georgia.

2 papers in the library · 18 citations · publishing 2024

Papers

Development of enantioselective high-performance liquid chromatography-tandem mass spectrometry method for the quantitative determination of 3,4-methylenedioxy-methamphetamine (MDMA) and its phase-1 metabolites in human biological fluids.

Journal of pharmaceutical and biomedical analysis January 20, 2024 Alfredo Fabrizio Lo Faro, Giorgia Sprega, Diletta Beradinelli et al. 14 citations

Enantioselective high-performance liquid chromatography-tandem mass spectrometry methods were developed to quantify MDMA and its phase-1 metabolites HMA, HMMA, and MDA in human plasma, sweat, oral fluid, and urine. Two polysaccharide-based chiral columns achieved baseline separation of most enantiomer pairs: the Lux AMP column separated MDMA, HMA, and HMMA enantiomers but not MDA; the Lux i-Amylose-3 column separated MDMA, HMMA, and MDA enantiomers but not HMA. Analysis took under 4 minutes with one column and under 6 minutes with the other. Both methods were validated and applied to biological fluids, confirming enantioselective metabolism of MDMA.

DOI: 10.1016/j.jpba.2023.115768 Full text Chiral hplc Enantioselective metabolism

Optimization of enantioselective high-performance liquid chromatography-tandem mass spectrometry method for the quantitative determination of 3,4-methylenedioxy-methamphetamine (MDMA) and its phase-1 metabolites in human biological fluids.

Journal of pharmaceutical and biomedical analysis June 15, 2024 Giorgia Sprega, Giorgi Kobidze, Alfredo Fabrizio Lo Faro et al. 4 citations

An improved chiral LC-MS/MS method separates all four pairs of enantiomers of MDMA and its major phase-1 metabolites (HMA, HMMA, MDA) on a single Lux AMP column within six minutes, using an optimized mobile phase and column dimensions. The method was applied to human plasma, oral fluid, and urine. In urine, hydrolysis of glucuronides with hydrochloric acid or glucuronidase was tested to evaluate effects on the concentration and enantiomeric distribution of the hydroxy metabolites HMA and HMMA.

DOI: 10.1016/j.jpba.2024.116076 Full text Chiral hplc Enantioselective metabolism Enantioseparation Hydrolysis of glucuronides +6