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John H. Krystal

Oak Ridge National Laboratory, United States Department of Veterans Affairs

29 papers in the library · 7,722 citations · publishing 1992-2026

Papers

Subanesthetic Effects of the Noncompetitive NMDA Antagonist, Ketamine, in Humans

Archives of General Psychiatry March 1, 1994 John H. Krystal 3,339 citations

Ketamine, a drug that blocks a specific brain receptor called NMDA, produces a broad set of effects in healthy people that resemble schizophrenia and dissociative states. In a randomized, double-blind, placebo-controlled study with 19 healthy adults, ketamine caused behaviors similar to both positive and negative symptoms of schizophrenia, altered perceptions, and impaired performance on tests of attention, verbal fluency, and cognitive flexibility. It disrupted delayed recall of words while sparing immediate and postdistraction recall. Ketamine also increased blood pressure and dose-dependently raised cortisol and prolactin levels, but did not affect a measure of general mental status. These findings suggest that NMDA receptor dysfunction may contribute to psychotic disorders.

Measurement of dissociative states with the Clinician‐Administered Dissociative States Scale (CADSS)

Journal of Traumatic Stress January 1, 1998 J. Douglas Bremner, John H. Krystal, Frank W. Putnam et al. 826 citations

A new instrument, the Clinician Administered Dissociative States Scale (CADSS), was developed to measure present-state dissociative symptoms. Initial analyses showed good interrater reliability and construct validity. CADSS scores successfully discriminated patients with dissociative disorders from patients with other psychiatric disorders and from healthy subjects.

Attenuation of the Neuropsychiatric Effects of Ketamine With Lamotrigine

Archives of General Psychiatry March 1, 2000 Amit Anand, Dennis S. Charney, Dan A. Oren et al. 425 citations

A drug that inhibits glutamate release, lamotrigine, reduced several effects of ketamine in healthy adults. Lamotrigine given before ketamine decreased perceptual abnormalities, positive and negative schizophrenia-like symptoms, and learning and memory impairment. However, it increased the immediate mood-elevating effects of ketamine. These findings suggest that glutamate release plays a role in some effects of NMDA receptor blockade and that drugs reducing glutamate release might help treat conditions like schizophrenia, though more research is needed.

Ketamine and Rapid-Acting Antidepressants: A Window into a New Neurobiology for Mood Disorder Therapeutics

Annual Review of Medicine October 23, 2014 Chadi G. Abdallah, Gerard Sanacora, Ronald S. Duman et al. 420 citations

Ketamine, a glutamate-based antidepressant, can rapidly alleviate depression within hours of treatment. Replicated evidence shows its rapid and potent effects in treatment-resistant depression. Preclinical and biomarker studies have begun to explain the mechanism behind these rapid effects, offering new insights into depression's biology and identifying potential treatment targets. This article discusses ketamine's efficacy, safety, and tolerability, summarizes depression's neurobiology, reviews the mechanisms of ketamine's rapid antidepressant effects, and considers prospects for next-generation rapid-acting antidepressants.

Changes in global and thalamic brain connectivity in LSD-induced altered states of consciousness are attributable to the 5-HT2A receptor

eLife October 25, 2018 Katrin H. Preller, Joshua B. Burt, Jie Lisa Ji et al. 416 citations

Lysergic acid diethylamide (LSD) reduces associative brain connectivity while increasing sensory-somatomotor and thalamic connectivity. These neural effects, along with the subjective experience, are fully blocked by ketanserin, a selective 5-HT2A receptor antagonist. The spatial pattern of LSD's effects across the brain matches the distribution of 5-HT2A receptor gene expression in humans. These results strongly implicate the 5-HT2A receptor in LSD's neuropharmacology, informing the neurobiology of psychedelics and guiding development of psychedelic-based therapeutics.

Comparative and Interactive Human Psychopharmacologic Effects of Ketamine and Amphetamine

Archives of General Psychiatry September 1, 2005 John H. Krystal, Edward Perry, Ralitza Gueorguieva et al. 323 citations

Ketamine and amphetamine produce different patterns of psychotic and cognitive effects in healthy people. Ketamine causes perceptual changes, negative symptoms, and memory disruption, while amphetamine triggers hostility, grandiosity, and somatic concern. Both drugs produce positive symptoms and euphoria, but their interaction reveals three patterns: amphetamine reduces ketamine-induced working memory impairment; the drugs additively increase thought disorder, arousal, and euphoria; and their combined effect on psychosis is less than additive. These results suggest that glutamate and dopamine systems contribute differently to psychosis, thought disorder, and euphoria, and that boosting prefrontal dopamine may help cognitive problems linked to glutamate dysfunction.

Linking Microcircuit Dysfunction to Cognitive Impairment: Effects of Disinhibition Associated with Schizophrenia in a Cortical Working Memory Model

Cerebral Cortex November 29, 2012 John D. Murray, Alan Anticevic, Mark G. Gancsos et al. 278 citations

Disruption of the balance between excitation and inhibition in the prefrontal cortex is thought to underlie cognitive problems in schizophrenia. A computational model of spatial working memory showed that disinhibition—caused by perturbing NMDA receptors on interneurons—broadens the tuning of memory-related neural activity, leading to more variable and less precise stored information and a reduced ability to filter out distractions. This prediction was tested with behavioral data from humans given ketamine, which induces disinhibition, and ketamine increased errors as the model predicted. The model also showed that restoring excitation-inhibition balance could reverse these working memory deficits, pointing to new experimental approaches for studying memory problems in schizophrenia.

NMDA receptor function in large-scale anticorrelated neural systems with implications for cognition and schizophrenia

Proceedings of the National Academy of Sciences September 25, 2012 Alan Anticevic, Mark G. Gancsos, John D. Murray et al. 260 citations

Glutamate signaling through NMDA receptors is essential for brain computations that support cognition, and its disruption may contribute to schizophrenia. Using ketamine, an NMDA receptor antagonist, the study found that the normal anticorrelation between the default-mode and task-positive brain systems was disrupted during a working memory task. The degree of this disruption predicted task performance and produced schizophrenia-like symptoms. A computational model suggests that cortical disinhibition underlies this effect, linking glutamate's role in large-scale brain organization to cognition and psychiatric symptoms.

NMDA receptor regulation of memory and behavior in humans

Hippocampus October 1, 2001 John W. Newcomer, John H. Krystal 238 citations

Reduced activity of NMDA receptors in the brain, which can be experimentally induced by antagonist drugs, impairs memory by disrupting the encoding and consolidation of information into long-term storage. More severe NMDA receptor hypofunction produces symptoms resembling psychosis and dissociation, and sustained underactivation triggers a neurotoxic process with distinct neuropathological features. As the brain ages, NMDA receptor function naturally declines, contributing to age-related memory and learning deficits. Preventing NMDA receptor hypofunction or its downstream effects may offer therapeutic strategies for memory and behavioral dysfunction in neuropsychiatric conditions.

KETAMINE'S MECHANISM OF ACTION: A PATH TO RAPID‐ACTING ANTIDEPRESSANTS

Depression and Anxiety April 6, 2016 Chadi G. Abdallah, Thomas G. Adams, Benjamin Kelmendi et al. 204 citations

Major depressive disorder is a common psychiatric condition that often responds poorly to traditional antidepressants, which can take weeks to work. Over the past two decades, the NMDA receptor antagonist ketamine has attracted attention because a single low dose produces rapid antidepressant effects in people with treatment-resistant depression. Evidence from animal and human studies suggests that ketamine triggers a surge of glutamate, initiating a cascade that promotes synaptogenesis and reverses stress-related damage, especially in the prefrontal cortex. This review covers the neurobiology of stress-related depression, the safety and efficacy of ketamine, its mechanism of action, and predictors of treatment response, along with research limitations and future directions.

Psilocybin Induces Time-Dependent Changes in Global Functional Connectivity

Biological Psychiatry January 13, 2020 Katrin H. Preller, Patricia Duerler, Joshua B. Burt et al. 199 citations

Psilocybin reduces connectivity in associative brain regions while increasing connectivity in sensory regions, a pattern that emerges over time from administration to peak effects. Baseline connectivity predicts the extent of these changes. The shifts correlate with spatial gene expression patterns of the serotonin 2A and 1A receptors, pinpointing their critical role in the psychedelic state. These findings suggest that sensory integration and associative disintegration may underlie the psychedelic experience, and baseline connectivity could serve as a predictive marker for personalized psychedelic treatment.

Chronic 3,4-Methylenedioxymethamphetamine (MDMA) Use: Effects on Mood and Neuropsychological Function?

The American Journal of Drug and Alcohol Abuse January 1, 1992 John H. Krystal, Lawrence H. Price, Charles Opsahl et al. 171 citations

Chronic use of MDMA (ecstasy) is associated with mild-to-moderate impairments in memory, as measured by the Wechsler Memory Scale, even when no memory deficits are apparent on clinical examination. In a study of nine individuals with extensive MDMA use, eight showed at least mild impairment on one or more neuropsychological tests. Despite previous evidence suggesting serotonin deficits in this group, none reported depressed mood or met criteria for an affective disorder at the time of testing. These findings raise concern about potential detrimental effects of MDMA on cognitive function and highlight important issues regarding serotonin's role in cognition and mood regulation.

Altered NMDA Glutamate Receptor Antagonist Response in Individuals With a Family Vulnerability to Alcoholism

American Journal of Psychiatry October 1, 2004 Ismene L. Petrakis, Diana Limoncelli, Ralitza Gueorguieva et al. 143 citations

A family history of alcoholism is linked to differences in how the brain responds to a drug that blocks NMDA glutamate receptors. Healthy young adults with at least two relatives who had ethanol dependence received intravenous infusions of a low or high dose of ketamine, an NMDA receptor antagonist, or a placebo. Compared to those with no family history of alcoholism, individuals with a family history reported fewer perceptual changes and less dysphoric mood during ketamine infusion. These findings suggest that inherited alterations in NMDA receptor function may influence the subjective effects of alcohol and contribute to the risk of developing alcoholism.

Ketamine as a promising prototype for a new generation of rapid‐acting antidepressants

Annals of the New York Academy of Sciences February 27, 2015 Chadi G. Abdallah, Lynnette A. Averill, John H. Krystal 117 citations

Ketamine produces rapid and robust antidepressant effects in patient populations that often show little or no response to traditional antidepressants, and it is generally well tolerated with only transient mild-to-moderate adverse effects during infusion. However, the optimal dosing, route of administration, and safety of chronic treatment remain uncertain. This review describes ketamine's clinical effects, its neurobiological mechanisms, and how these may illuminate the neurobiology of depression, relevant biomarkers, and treatment targets. The authors also suggest directions for future research to improve psychopharmacologic interventions for depressive and trauma-related disorders.

Long-term safety of ketamine and esketamine in treatment of depression

Expert Opinion on Drug Safety April 13, 2022 Sina Nikayin, Eva Murphy, John H. Krystal et al. 114 citations

Ketamine and its derivative esketamine (Spravato) can rapidly relieve depression, but their long-term safety is reviewed here. Common side effects are temporary and mild, including dissociation, nausea, headache, and changes in heart rate and blood pressure. Esketamine may increase lower urinary tract symptoms, but severe bladder problems have not occurred at prescribed depression doses. High-dose ketamine can impair cognition long-term, but esketamine trials show cognition remains stable or improves, indicating no increased cognitive risk with appropriate use.

Ketamine Effects on Memory Reconsolidation Favor a Learning Model of Delusions

PLoS ONE June 12, 2013 Philip R. Corlett, Victoria C. Cambridge, Jennifer M. Gardner et al. 104 citations

Delusions, the persistent bizarre beliefs characteristic of psychosis, may arise from disturbances in prediction error-dependent learning. In a placebo-controlled study with 18 human subjects, ketamine—an NMDA receptor antagonist that induces aberrant prediction error signals—was administered during re-exposure to a conditioned fear stimulus. This led to stronger subsequent fear memory compared to placebo, with the degree of strengthening correlating with individual vulnerability to ketamine's psychotogenic effects and with prediction error brain signals. A partial replication in an independent sample with an appetitive learning procedure (8 subjects) supported these findings. The results suggest a link between altered prediction error, memory strength, and psychosis, potentially explaining both the emergence and persistence of delusional beliefs.

Transcriptomics-informed large-scale cortical model captures topography of pharmacological neuroimaging effects of LSD

eLife July 12, 2021 Joshua B. Burt, Katrin H. Preller, Murat Demirtaş et al. 49 citations

A computational model that simulates how LSD affects human brain activity shows that the drug alters communication between cortical areas by increasing the sensitivity of pyramidal neurons via the serotonin-2A receptor. The model accurately reproduced changes in functional connectivity observed in brain scans, and fitting it to individual participants captured personal differences in drug response related to altered consciousness. This approach links molecular drug actions to large-scale brain network changes, offering a path toward personalized medicine.

Recent Advances in the Treatment of Treatment-Resistant Depression: A Narrative Review of Literature Published from 2018 to 2023

Current Psychiatry Reports April 1, 2024 John L. Havlik, Syed Wahid, Kayla M. Teopiz et al. 30 citations

Treatment-resistant depression (TRD) has historically had very limited options, but recent advances have expanded knowledge of effective interventions. Psychotherapy can help as an add-on but not alone. Adjunctive non-antidepressant drugs like buprenorphine and antipsychotics show little recent support; side effects and high discontinuation rates may outweigh benefits. Strong recent evidence supports interventional approaches: electroconvulsive therapy, ketamine/esketamine, and transcranial magnetic stimulation. Research on TRD should use internationally defined inclusion criteria for generalizable results.

Psilocybin: From Psychiatric Pariah to Perceived Panacea

American Journal of Psychiatry January 1, 2025 Adrienne Grzenda, Gregory A. Fonzo, Aaron Wolfgang et al. 14 citations

Current evidence does not support recommending psilocybin combined with psychological support (PST) as a psychiatric treatment. More rigorous clinical trials are needed to confirm its effectiveness in larger and more diverse patient groups, determine appropriate dosing, improve blinding methods, and understand how it works and for whom it works best. Comparing it directly with other proven treatments will clarify its potential future role in treating major psychiatric disorders.

Ketamine for Depression, but at What Cost? A Review of Ketamine’s Neurotoxic Effects From Preclinical and Human Studies

American Journal of Psychiatry September 10, 2025 Songze Li, Kristina T. Kumpf, Julian Urrutia et al. 13 citations

Repeated or high-dose subanesthetic ketamine causes excitotoxic neuronal damage and lasting cognitive deficits in animals, especially perinates. Infrequent low-to-moderate doses (<1 mg/kg human intravenous equivalent) do not produce overt brain damage in rats and nonhuman primates. In humans, frequent high-dose (>1 g/day) recreational users show memory and executive function impairments. However, a large clinical trial found that intranasal esketamine at up to 84 mg weekly or every other week for several years is associated with maintained or slightly improved cognition in adults with major depression, though some elderly patients showed potential worsening in attention and processing speed. Direct comparisons of esketamine and off-label racemic ketamine at higher doses are lacking.

Psychotherapy-supported MDMA treatment for PTSD

Cell Reports Medicine August 1, 2021 John H. Krystal, Benjamin Kelmendi, Ismene L. Petrakis 13 citations

A Phase III trial of MDMA combined with psychotherapy for severe PTSD produced a 24.4-point (55.5%) reduction in PTSD severity, compared with 13.9 points (31.5%) for placebo, and 33% of the MDMA group achieved remission versus 5% of the placebo group. The effect size was large (d > 0.9). MDMA also improved depression and social disability. The commentary notes the study was small for a Phase III trial, raises concerns about unblinding due to MDMA's subjective effects, and calls for larger replication trials, active comparators, and better safety monitoring for neurotoxicity and addiction liability.

Modulating amygdala activation to traumatic memories with a single ketamine infusion

medRxiv Preprint Server July 7, 2021 Or Duek, Yutong Li, Ben Kelmendi et al. 8 citations preprint

A single low-dose infusion of ketamine, an NMDA receptor antagonist, given after recalling a traumatic memory can weaken the fear response associated with post-traumatic stress disorder. In the study, people who received ketamine showed lower activity in the amygdala and hippocampus when re-exposed to trauma memories, compared to those who received midazolam. Ketamine also reduced communication between the amygdala and hippocampus, without affecting connections to the prefrontal cortex. These changes lasted at least 30 days after treatment, suggesting that human traumatic memories can be altered during a reconsolidation window, potentially offering a new approach to treating PTSD.

Ketamine Effects on Energy Metabolism, Functional Connectivity and Working Memory in Healthy Humans

bioRxiv Preprint Server February 21, 2023 Naomi R. Driesen, Peter Herman, Margaret A. Rowland et al. 5 citations preprint

Ketamine, an NMDAR antagonist, increased oxidative metabolism (CMRO2) and cerebral blood flow in the prefrontal cortex and other cortical regions, but did not alter resting-state cortical functional connectivity or brain-wide CBF-CMRO2 coupling. Higher baseline CMRO2 was associated with lower task-related prefrontal activation and greater working memory accuracy impairment under both saline and ketamine conditions. These findings suggest that CMRO2 and resting-state functional connectivity index distinct dimensions of neural activity, and that ketamine's impairment of working memory-related neural activity and performance relates to its induction of cortical metabolic activation.

Ketamine Alters Tuning of Neural and Behavioral Spatial Working Memory Precision

bioRxiv Preprint Server February 10, 2025 Masih Rahmati, Flora Moujaes, Nina Purg Suljič et al. 1 citation preprint

Working memory deficits in disorders like schizophrenia may stem from disrupted brain cell tuning. Using fMRI, researchers found that ketamine, which blocks NMDA receptors, broadens neural spatial tuning in healthy people, reducing the precision of brain responses across visual, parietal, and frontal areas and worsening spatial working memory accuracy. These tuning changes were more consistent across individuals and brain regions than overall activation changes and correlated with memory performance. The results link NMDA receptor disruption to altered brain circuit dynamics and memory impairment, offering a target for developing treatments.