Metabolites
November 12, 2021
Jitka Nykodemová, Anna Šuláková, Petr Palivec et al.
14 citations
The metabolism of the psychoactive compound 2C-B-Fly-NBOMe was investigated using three systems: human liver microsomes, the fungus Cunninghamella elegans, and live rats. Thirty-five phase I and nine phase II metabolites were identified. Major metabolic pathways include hydroxylation, O-demethylation, oxidative debromination, and N-demethoxybenzylation, followed by glucuronidation or N-acetylation. Human liver microsomes produced the most metabolites at highest concentrations. Two poly-hydroxylated metabolites appeared only in rat urine, while the fungus generated dehydrogenated, N-oxygenated, and dibrominated metabolites. These findings clarify how the body processes this substance, aiding understanding of its effects and potential toxicity.
Metabolites
March 31, 2021
Anna Šuláková, Jitka Nykodemová, Petr Palivec et al.
11 citations
N-Benzylphenethylamines, including 25CN-NBOMe, are novel psychedelic substances with limited metabolism data. This study investigated the metabolic profile of 25CN-NBOMe in rats in vivo and in human liver microsomes and Cunninghamella elegans mycelium in vitro. Major metabolic pathways include mono- and bis-O-demethylation, hydroxylation, and combinations, followed by glucuronidation, sulfation, or N-acetylation of primary metabolites. The cyano group was either hydrolyzed to an amide or carboxylic acid or remained unchanged. Differences between species should be considered in metabolism studies of novel substances.
Journal of pharmacological and toxicological methods
January 1, 2019
Anna Šuláková, Lucie Fojtíková, Barbora Holubová et al.
11 citations
Two new immunoassays, a lateral flow immunoassay (LFIA) and an enzyme-linked immunosorbent assay (ELISA), were developed to detect the hallucinogenic phenethylamine 2C-B and related drugs in urine. The LFIA provides rapid on-site screening with a detection limit of 15 ± 7 ng mL⁻¹, suitable for identifying recent exposure. The ELISA offers much higher sensitivity, with a detection limit of 6 ± 3 pg mL⁻¹, and its results closely match those from established UHPLC-MS-MS analysis, making it reliable for quantitative confirmation in toxicology. Both methods address the need for simple, cost-effective tools to monitor these dangerous substances.
Therapeutic advances in psychopharmacology
January 1, 2026
Veronika Andrashko, Tomáš Novák, Miloslav Kopecek et al.
Higher blood pressure during ketamine infusion was associated with better antidepressant response in people with depression. Among 39 patients who received a single intravenous dose of ketamine, those who responded at day 7 had significantly higher systolic and diastolic blood pressure during the infusion than nonresponders. Dissociation and psychotomimetic symptoms did not differ between responders and nonresponders. Concomitant antipsychotic medication was linked to worse antidepressant outcome and lower infusion-related blood pressure. Plasma levels of ketamine or norketamine did not correlate with blood pressure, dissociative symptoms, or psychotomimetic effects.