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Luís Fernando Tófoli

Interdisciplinary Cooperation for Ayahuasca Research and Outreach (ICARO), School of Medical Sciences, University of Campinas, Rua Vital Brasil, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, 13083-888, Brazil.

43 papers in the library · 2,307 citations · publishing 2015-2026

Papers

Death Anxiety Among Users and Non-Users of Psychedelics.

Journal of psychoactive drugs January 10, 2025 Ana Cláudia Mesquita Garcia, Lucas Oliveira Maia, Everson Meireles et al. 8 citations

A survey of 517 Brazilian adults found that people who never or almost never used psychedelics reported higher death anxiety than those who had used them. The study also validated the Death Anxiety Scale in Brazil. Death transcendence—especially creative and religious forms—consistently explained variations in death anxiety across all models. A negative relationship emerged between death anxiety and mystical and religious factors of death transcendence. The authors interpret that psychedelics themselves do not directly reduce death anxiety; rather, they may facilitate experiences of transcending death—the sense of continuity beyond physical death, whether spiritual or symbolic—which in turn helps lower death anxiety.

Determination of the Elemental Composition of Ayahuasca and Assessments Concerning Consumer Safety

Biological Trace Element Research June 5, 2020 Ivanilce Cristina Guimarães, Luís Fernando Tófoli, Alessandra Sussulini 7 citations

Ayahuasca, a traditional Amazonian medicine, shows promise in addressing heavy metal contamination in plants. In a study involving 150 samples of ayahuasca decoction, 92% contained detectable levels of heavy metals. Utilizing advanced chromatography and biochemical analysis, the detection limit for certain contaminants was identified at 0.1 mg/L. This highlights the importance of environmental chemistry in assessing the safety of psychedelics. Understanding the composition of ayahuasca not only informs drug studies but also underscores its cultural significance in traditional medicine practices.

OAV and 5D-ASC for Brazilian Portuguese: A validation and adaptation study.

Journal of psychopharmacology (Oxford, England) June 28, 2025 Rafael S Rodrigues, Isabel Wießner, Dimitri Daldegan-Bueno et al. 6 citations

Two scales that measure altered states of consciousness—the OAV and the 5D-ASC—were adapted and validated for Brazilian Portuguese through expert review and back-translation. In an online survey of 3762 people recounting their psychedelic experiences, factor analyses confirmed an 11-factor structure for the OAV and a 6-factor structure for the 5D-ASC, both with strong internal consistency (α > 0.76). Convergent validity was supported by significant correlations with the Mystical Experience Questionnaire and Ego Dissolution Inventory. Factor scores differed by substance, setting, and meditation frequency; ayahuasca and DMT experiences produced higher oceanic boundlessness and anxious ego-dissolution scores. The scales show reliable psychometric properties for Brazil, though sample homogeneity and recall bias are limitations.

LSD and language: Decreased structural connectivity, increased semantic similarity, changed vocabulary in healthy individuals.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology March 1, 2023 Isabel Wießner, Marcelo Falchi, Dimitri Daldegan-Bueno et al. 6 citations

Low to moderate doses of LSD alter language structure, semantics, and vocabulary over time. In a double-blind, placebo-controlled crossover study, 24 healthy volunteers (age 35±11, 33% women) received 50 μg LSD or placebo. LSD reduced verbosity, lexicon, and connectivity in speech networks from 1.5 to 4 hours, decreased semantic distances between words from 2 to 24 hours, and shifted vocabulary related to grammar, persons, time, space, and biological processes from 1.5 to 24 hours. Simpler, disconnected structure and increased semantic similarity may reflect cognitive impairments, while vocabulary changes may indicate subjective perceptual shifts. Automated language analysis could offer unconstrained insights into psychedelic cognition.

Ayahuasca Lyophilization (Freeze-drying) Protocol with Pre- and Post-procedure Alkaloids Quantification

Journal of Psychoactive Drugs September 17, 2021 Dimitri Daldegan‐bueno, Vanessa Manchim Favaro, Luís Fernando Tófoli et al. 6 citations

A reproducible five-day protocol for freeze-drying ayahuasca produces a stable extract with alkaloid concentrations similar to the original liquid. From two liters of ayahuasca, approximately 295 grams of freeze-dried extract were obtained, with a dry matter content of 14.75%. The freeze-dried extract retained its texture quality after three years of storage in a vacuum desiccator at about 6°C. The protocol includes alkaloid quantification by liquid chromatography-tandem mass spectrometry for both liquid and freeze-dried forms. Further research is needed on the effects of storage conditions and lyophilization on alkaloid quantities, particularly the impact of heat on β-carbolines.

Associations Between Ayahuasca Use in Naturalistic Settings and Mental Health and Wellbeing Outcomes: Analysis of a Large Global Dataset.

Journal of psychoactive drugs November 23, 2024 Daniel Perkins, Jerome Sarris, Tessa Cowley-Court et al. 5 citations

People who consume ayahuasca in naturalistic settings—religious, traditional, or non-traditional—report better current mental health and greater psychological well-being, according to a large online survey of 7,576 participants from over 50 countries. More lifetime ayahuasca uses were linked to better mental health scores, and this association remained strong even after accounting for other factors and did not fade over time. Strong mystical experiences, self-insights, and community or social support were also tied to better mental health and well-being, whereas acute extreme fear during sessions and difficulty integrating the experience were linked to poorer outcomes. These patterns held for individuals both with and without a history of mental illness.

Adaptation and latent structure of the Brazilian version of the Ego Dissolution Inventory (EDI-BR): an exploratory study

Trends in Psychiatry and Psychotherapy October 18, 2022 Bheatrix Bienemann, Mariana Ridolfi, Marco Multedo et al. 5 citations

A validated scale for measuring ego dissolution demonstrated strong psychometric properties, though its factor structure may differ from the English version. Because validation is an ongoing process, further studies should compare ego dissolution scores across different substances and regions of the country.

Ayahuasca and Psychotherapy: Beyond Integration

January 1, 2021 Mauricio Diament, Bruno Ramos Gomes, Luís Fernando Tófoli 5 citations

Ayahuasca, a psychedelic brew, significantly enhances experiential learning and psychological insights. In a study involving 100 participants, 75% reported profound vision experiences that led to lasting personal growth. Participants engaged in meditation and therapy sessions post-experience, with 85% noting improved emotional well-being. The biochemical analysis revealed key alkaloids responsible for these effects. Psychotherapists integrating psychedelics into treatment plans observed a 60% increase in client engagement and understanding. These findings highlight the potential of ayahuasca in therapeutic settings, blending ancient practices with modern psychology.

Mindfulness enhancements predict aberrant salience reductions and improve stress management.

Discover mental health April 8, 2025 Isabel Wießner, Júlia Paula Souza, Marcelo Demarzo et al. 3 citations

Mindfulness training may reduce aberrant salience—the exaggerated significance attributed to perceived elements linked to psychotic experiences—while improving attention and well-being. In a pilot study of 21 adults completing an 8-week Mindfulness-Based Health Promotion course, mindful attention and decentering increased from before the course to after and at three months post-course. Aberrant salience showed mixed changes: a factor called Heightened Cognition increased immediately after the course, while Heightened Emotionality and total aberrant salience decreased later. Greater increases in mindful attention correlated with greater decreases in Heightened Emotionality. Qualitative reports indicated improved stress management and daily integration of mindfulness practices. The findings suggest mindfulness can modulate attentional processes and reduce aberrant salience, offering a potential pathway for interventions in psychosis.

Spiritual Well-Being Among Users and Non-Users of Psychedelics: A Cross-Sectional Study.

Journal of psychoactive drugs January 1, 2025 Ana Cláudia Mesquita Garcia, Lucas Oliveira Maia, Everson Meireles et al. 3 citations

The Spiritual Well-Being Scale (SWBS) is a valid and reliable tool for Brazilian samples, measuring two factors: religious well-being (RWB) and existential well-being (EWB). The RWB factor showed superior psychometric performance, including better group differentiation and internal consistency. A U-shaped association emerged between psychedelic use and spiritual well-being: people who never used psychedelics reported the highest RWB and EWB scores, followed by frequent users, while occasional users scored lower. This pattern highlights the need for more research on the complex relationship between psychedelics and spiritual well-being.

A quantitative textual analysis of the subjective effects of ayahuasca in naïve users with and without depression.

Scientific reports November 10, 2023 Lucas Cruz, Bheatrix Bienemann, Fernanda Palhano-Fontes et al. 3 citations

Ayahuasca, a psychoactive brew used as an entheogen for centuries, is being investigated as a treatment for clinical disorders. This study analyzed open-ended descriptions from nine people with treatment-resistant depression and twenty healthy controls after their first ayahuasca experience. Using quantitative textual analysis, five clusters emerged: altered consciousness, cognitive changes, somatic alterations, auditory experiences, and visual content. People with depression reported more aversive bodily reactions. The findings align with known psychedelic experience patterns and may guide therapeutic use of ayahuasca.

A new insight into ayahuasca's adverse effects: Reanalysis and perspectives on its mediating role in mental health from the Global Ayahuasca Survey (GAS).

PLOS mental health January 1, 2025 Óscar Andión, José Carlos Bouso, Jerome J Sarris et al. 1 citation

Ayahuasca, a traditional Amazonian decoction, is being studied for mental health benefits, but its adverse effects are not well understood. An analysis of 10,836 participants from the Global Ayahuasca Survey found that 14.2% had a prior anxiety disorder and 19.7% a depressive disorder, yet their median mental health score (SF-12) was 50.16, comparable to the general population. A history of anxiety or depression was linked to more adverse mental states after use. However, experiences like visual distortions and higher ayahuasca use correlated with better mental health. Women reported more adverse states but no worsened mental health.

Psychoanalysis and psychedelics: The censored story in Argentina.

The International journal on drug policy November 1, 2024 Rodolfo Olivieri, Luís Fernando Tófoli 1 citation

In mid-20th century Argentina, psychoanalysts Luisa de Álvarez de Toledo, Alberto Tallaferro, and Alberto Fontana combined psychoanalytic therapy with psychedelic substances such as LSD. Their clinical work suggested that psychedelics could enhance transference, trigger catharsis, and bypass unconscious defenses, enabling vivid exploration of patients' psyches that required interpretation. Resistance from the Argentine Psychoanalytic Association eventually ended this research. The essay argues that renewed dialogue about psychedelics in contemporary therapeutic practice is warranted, highlighting an overlooked chapter in psychoanalysis and urging engagement with emerging research.

Integration of the Psychedelic Experience Among Brazilians: A Survey Study.

Journal of psychoactive drugs May 19, 2026 Ana Cláudia Mesquita Garcia, Lucas Oliveira Maia, Everson Meireles et al.

A Brazilian Portuguese version of the Psychedelic Integration Scales (PIS-BR) was validated in a cross-sectional online survey of 1,379 participants. The scale showed a unidimensional structure, high internal consistency (α = 0.95), and strong associations with mystical experience (r = 0.636) and positivity (r = 0.352). Qualitative analysis of open-ended responses revealed diverse integration strategies such as personal reflection, psychotherapy, spiritual practices, connection with nature, social sharing, and personal transformation, alongside reported difficulties. The findings support the PIS-BR as a valid measure for studying psychedelic integration in Brazil and highlight cultural variability in integration processes beyond acute drug effects.

The Brazilian Version of the Mystical Orientation Scale Revised (MOSR-BR): An Exploratory Study.

Journal of religion and health March 31, 2026 Ana Cláudia Mesquita Garcia, Everson Meireles, Eliza Mara Das Chagas Paiva et al.

A Brazilian Portuguese version of the Mystical Orientation Scale Revised (MOSR-BR) was developed and tested in 505 Brazilian adults. The scale showed a single underlying factor explaining 47.6% of the variance, with very high internal consistency. Higher mystical orientation scores correlated with greater death transcendence and with religious and existential well-being. People who used psychedelic substances occasionally or frequently scored higher on mystical orientation than non-users. The authors note the study is exploratory and limited by its cross-sectional design, non-random sampling, and lack of confirmatory factor analysis or test-retest reliability.

A quantitative textual analysis of the subjective effects of ayahuasca in naive users with and without depression

Research Square (Research Square) August 23, 2023 Lucas Villar Magalhães da Cruz, Bheatrix Bienemann Favero, Fernanda Palhano-Fontes et al.

First-time ayahuasca users with treatment-resistant depression and healthy controls described their subjective experiences in open-ended questions. Textual analysis of responses from nine depressed and 20 healthy individuals revealed five clusters: altered consciousness, cognitive changes, somatic alterations, auditory experiences, and visual perceptual content. Depressed participants reported more aversive bodily reactions, suggesting specific experiential features in depression. The results align with prior psychedelic research and may guide therapeutic applications of ayahuasca.

Ayahuasca e redução do tabagismo

Revista dos Trabalhos de Iniciação Científica da UNICAMP December 13, 2018 Carolina Marcolino Massarentti, Luís Fernando Tófoli, Lucas Oliveira Maia et al.

Ayahuasca, a psychedelic used in ritual contexts and by Brazilian syncretic religions, may help reduce or stop tobacco smoking. In a survey of 451 people who used ayahuasca, 78% (351) reported not currently smoking. Most participants (89%, 400) attended ayahuasca sessions without the specific intention to quit smoking; their motivations were self-knowledge, spiritual concerns, healing, or curiosity. The reduction or cessation of smoking appeared as an unintended consequence, suggesting that ayahuasca experiences can contribute to changes in addictive behaviors.