Development of an enzyme immunoassay using a monoclonal antibody against the psychoactive diterpenoid salvinorin A.
Journal of natural products September 27, 2013 Madan Kumar Paudel, Osamu Shirota, Kaori Sasaki-Tabata et al. 21 citations
A monoclonal antibody was created that recognizes salvinorin A, the main psychoactive compound in Salvia divinorum, and an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to detect salvinorins. The assay's calibration range was 0.0195-0.625 μg/mL. Tests on plants from the mint family, including S. divinorum, showed the method is simple, precise, accurate, sensitive, and reliable for identifying the plant.