LC-MS/MS screening method for designer amphetamines, tryptamines, and piperazines in serum.
Analytical and bioanalytical chemistry April 1, 2010 Ariane Wohlfarth, Wolfgang Weinmann, Sebastian Dresen 128 citations
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method can simultaneously identify 35 substances, including 31 new designer drugs plus cathinone, methcathinone, phencyclidine, and ketamine. The method covers amphetamine derivatives (e.g., 2C-B, DOB, PMA), tryptamine derivatives (e.g., DMT, 5MeO-DMT), and piperazine derivatives (e.g., BZP, mCPP). After solid-phase extraction and gradient elution, detection limits range from 1.0 to 5.0 ng/mL, matrix effects from 65% to 118%, and extraction efficiencies from 72% to 90%. The assay is selective for all tested substances.