A new screening method using solid-phase extraction and liquid chromatography with photodiode array detection can simultaneously identify and quantify thirteen plant alkaloids in human samples. The validated technique provides selective, accurate, and sensitive measurements of these compounds, which include both toxic and medicinal substances. Analysis of forensic and clinical specimens demonstrates the method's usefulness for detecting intoxications, monitoring drug abuse, and checking patient compliance with prescribed medications.
After oral (25 mg fruit gum) or inhalative (three puffs from a vape) consumption of the semi-synthetic cannabinoid hexahydrocannabinol (HHC), its pharmacokinetics and psychoactive effects are comparable to those of tetrahydrocannabinol (THC). The route of administration and inter-individual factors strongly influence maximum concentrations, pharmacokinetic profiles, and the subjective 'high' feeling. Serum, urine, and saliva samples were analyzed for HHC and its metabolites. Three commercially available immunological tests were also evaluated for detectability.