Simultaneous determination of thirteen plant alkaloids in a human specimen by SPE and HPLC.
Jörg Pietsch, Jana Günther, Thomas Henle, Jan Dressler
Journal of separation science July 1, 2008 DOI: 10.1002/jssc.200800128 via PubMed
Summary
AI-generated from the abstractA new screening method using solid-phase extraction and liquid chromatography with photodiode array detection can simultaneously identify and quantify thirteen plant alkaloids in human samples. The validated technique provides selective, accurate, and sensitive measurements of these compounds, which include both toxic and medicinal substances. Analysis of forensic and clinical specimens demonstrates the method's usefulness for detecting intoxications, monitoring drug abuse, and checking patient compliance with prescribed medications.
Study at a glance
| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Population | Human specimens (forensic and clinical samples) |
| Keywords | Analytical methods Toxicology Forensic science Clinical diagnostics |
| Citations | 56 |
| Key finding | A validated solid-phase extraction and liquid chromatography method enables simultaneous detection and quantification of thirteen plant alkaloids in human specimens for forensic and clinical applications. |
Abstract
A new screening method for the simultaneous determination of thirteen plant alkaloids (aconitine, anabasine, atropine, brucine, colchicine, cotinine, cytisine, harmine, ibogaine, nicotine, scopolamine, strychnine, yohimbine) in a human specimen was developed based on solid-phase extraction and reversed-phase liquid chromatography with photodiode array detection. The validated method enables selective identification as well as accurate and sensitive quantification. The analysis of forensic and clinical samples emphasizes the applicability for intoxications and drug abuse, as well as for compliance control.