Liquid chromatography-tandem mass spectrometry method for the simultaneous analysis of multiple hallucinogens, chlorpheniramine, ketamine, ritalinic acid, and metabolites, in urine.
Journal of analytical toxicology October 1, 2007 Maria Del Mar Ramirez Fernandez, Marleen Laloup, Michelle Wood et al. 47 citations
A method for simultaneously measuring multiple hallucinogens, chlorpheniramine, ketamine, ritalinic acid, and several metabolites in human urine was developed and validated. The procedure uses solid-phase extraction followed by liquid chromatography-tandem mass spectrometry, with all drugs eluting within 14 minutes. Using 500 microliters of urine, limits of quantification ranged from 0.05 ng/mL for LSD to 10 ng/mL for other hallucinogens, with linear or quadratic regression from each compound's limit up to 500 ng/mL. Precision and accuracy were acceptable, and processed samples remained stable in the autosampler for at least 24 hours. The method was successfully applied to authentic urine samples containing chlorpheniramine, ketamine, LSD, and psilocin.