In vivo determination of 5-hydroxytryptamine receptor-stimulated phosphoinositide turnover in rat brain.
Journal of neurochemistry August 1, 1989 I Hide, T Kato, S Yamawaki 15 citations
In the intact rat brain, phosphoinositide turnover is stimulated by activation of the 5-HT2 receptor. After prelabeling brain phosphoinositides with [3H]inositol, lithium treatment increased levels of [3H]IP1 and [3H]IP2. The 5-HT agonists 5-MeODMT and quipazine further increased 3H-inositol phosphate levels under lithium pretreatment. This response was blocked by the 5-HT2 antagonist ritanserin but not by the 5-HT1 antagonist (-)-propranolol, indicating that 5-HT2 receptors drive this signaling. The method can be used to study in vivo effects of psychotropic drugs like antidepressants on 5-HT2 receptor-stimulated phosphoinositide turnover.