Determination of 5-OH-DMT (bufotenine) in the Asian toad Duttyphrynus melanostictus, Hoplobatrachus tigerinus and Haemopis sanguisiga in Madagascar by UHPLC-MS/MS.
Environmental toxicology and pharmacology April 1, 2025 Christina Ververi, Elisah Rasoanomenjanahary Nahavitatsara, Brigitte Annie Bezandry et al.
Bufotenine, a psychoactive compound, was detected in the skin of the invasive toad Duttaphrynus melanostictus in Toamasina, Madagascar, and in its predators, the tiger frog Hoplobatrachus tigerinus and the leech Haemopis sanguisiga. Concentrations increased with the toad's developmental stage; eggs contained no detectable bufotenine, while tadpoles and adult skin did. A simple liquid-liquid extraction and UHPLC-MS/MS method reliably quantified the compound across a 10–1000 ng/mL range.