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Rui-Lin Zhao

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

5 papers in the library · 6 citations · publishing 2024-2026

Papers

Five New Species of Gymnopilus from Xizang Autonomous Region of China and Surrounding Areas

Journal of Fungi March 18, 2024 Wen-Qiang Yang, Mao-Qiang He, Dorji Phurbu et al. 3 citations

A molecular phylogenetic and morphological study of the wood-decaying mushroom genus Gymnopilus examined seventy-eight specimens collected from ten provinces in China. Using gene sequences from ITS, nrLSU, nrSSU, rpb1, rpb2, and tef1-α, researchers identified eleven species, including five new to science: Gy. gyirongensis, Gy. variisporus, Gy. tomentosiceps, Gy. tenuibasidialis, and Gy. aurantipileatus. Four of the five new species are native to the Xizang Autonomous Region, indicating specialization to that distinctive habitat. The work advances understanding of Gymnopilus diversity and provides a foundation for conservation and sustainable use of these fungi.

Molecular Phylogeny and Morphology Reveal Four New Species of Conocybe (Bolbitiaceae, Agaricales) from the Qinghai-Xizang Plateau, China

Journal of Fungi January 7, 2025 Xi-Xi Han, Dorji Phurbu, Bin Cao et al. 2 citations

On the Qinghai-Xizang Plateau, a region shaped by high altitude, plate collision, and crustal uplift, seven Conocybe mushroom species were identified from specimens collected over the past decade. Four of these—C. alticola, C. alticoprophila, C. versicolor, and C. yadongensis—are new to science. Multi-gene DNA sequencing (ITS, nrLSU, and tef-1α) confirmed their taxonomic placement within the genus. Detailed descriptions, illustrations, and comparisons with related species are provided. This work expands knowledge of Conocybe diversity on the plateau, contributing to understanding fungal biodiversity in extreme environments. Some Conocybe species produce psilocybin and other compounds with potential medicinal value.

Evolution and horizontal transfer of the psilocybin biosynthetic gene cluster drive the diversification of magic mushrooms

Mycosphere December 25, 2025 Fei Liu, Jiaxin Li, Wen-Qiang Yang et al. 1 citation

Psilocybin, the psychoactive compound in magic mushrooms, is made by a biosynthetic gene cluster (BGC) once thought unique to Psilocybe species but now found across multiple genera. Sequencing genomes of 30 psilocybin-producing species and comparing them with over 20,000 bacterial, plant, and fungal genomes suggests the BGC originated from endogenous fungal genes through duplication and rearrangement, not from horizontal gene transfer from nonfungal sources. Four independent horizontal transfer events and three BGC configurations were identified. Transcriptomic analysis showed high expression of the PsiK gene in mycelium, while PsiH and PsiM were inactive, matching the absence of psilocybin in mycelial tissue. The BGC's evolution, along with a coprophilous (dung-inhabiting) habit, points to a post-Cretaceous-Tertiary radiation linked to the rise of mammals and grasslands.

Systematic study of Panaeolus (Agaricales, Galeropsidaceae) sensu lato and psilocybin-producing traits of species from China.

IMA fungus January 1, 2026 Mao-Qiang He, Wen-Qiang Yang, Dorji Phurbu et al.

The hallucinogenic mushroom group Panaeolus sensu lato, commonly found on dung and in grasslands, is shown through phylogenomic, multigene phylogenetic, molecular clock, and morphological analyses to form a distinct family, Galeropsidaceae, separate from Bolbitiaceae. The family's taxonomy is revised: accepted genera are Panaeolus and Staktophyllus, with Crucispora and Panaeolopsis synonymized under Panaeolus. Three subgenera are recognized in Panaeolus, including one new subgenus, and eight new species are proposed. Psilocybin production was tested in 14 species using HPLC-MS; two species—the known P. cinctulus and the new P. subfoenisecii—are confirmed to produce psilocybin. The evolution of dung-inhabiting and psilocybin-producing traits in Panaeolus is discussed using phylogenetic relationships and divergence times.

Systematic study of Panaeolus (Agaricales, Galeropsidaceae) sensu lato and psilocybin-producing traits of species from China

Global Biodiversity Information Facility January 1, 2026 Mao-Qiang He, Wen-Qiang Yang, Dorji Phurbu et al.

A group of hallucinogenic mushrooms, Panaeolus sensu lato, is shown to belong to a distinct family, Galeropsidaceae, separate from Bolbitiaceae, based on phylogenomic, multigene phylogenetic, molecular clock, and morphological analyses. The taxonomic system of Galeropsidaceae is revised: it contains the genera Panaeolus and Staktophyllus, while Crucispora and Panaeolopsis are synonymized under Panaeolus. Three subgenera are accepted in Panaeolus. Eight new species and one new subgenus are proposed. Species from China are documented with descriptions and illustrations. Testing 14 species for psilocybin using HPLC–MS confirmed that the known species P. cinctulus and the new species P. subfoenisecii possess psilocybin-producing traits. The evolution of the coprophilous lifestyle and psilocybin-producing traits is discussed.