Enzyme‐assisted synthesis of the glucuronide conjugate of psilocin, an hallucinogenic component of magic mushrooms
Drug Testing and Analysis February 2, 2011 Takuji Shoda, Kiyoshi Fukuhara, Yukihiro Goda et al. 8 citations
An enzyme-assisted method produced psilocin glucuronide (PCG), a metabolite excreted in the urine of magic mushroom users. Using Aroclor 1254 pretreated rat liver microsomes, psilocin and the cofactor UDPGA were incubated for 20 hours. HPLC purification yielded 3.6 mg of PCG (19% yield). The structure was confirmed by mass spectrometry and NMR. The milligram amounts produced will enable direct identification and quantification of PCG in the urine of magic mushroom users.