Repeated microdoses of LSD do not alter anxiety or boldness in zebrafish.
Scientific reports February 22, 2024 Ethan V Hagen, Melike Schalomon, Yanbo Zhang et al. 11 citations
LSD at low concentrations (1.5, 15, and 150 µg/L) acutely reduced high mobility in zebrafish, and the two lowest concentrations also decreased swimming velocity. However, after repeated daily exposure for ten days followed by a seven-day withdrawal period, no significant differences in anxiety-like behavior, boldness, or locomotion were observed between any LSD-treated group and controls. These results suggest that while LSD can produce acute behavioral effects at very low doses, tolerance may develop with repeated administration, and no lasting behavioral changes persist after withdrawal.