Screening and confirmation of psilocin, mitragynine, phencyclidine, ketamine and ketamine metabolites by liquid chromatography-tandem mass spectrometry.
Journal of analytical toxicology March 1, 2024 Madeleine E Wood, Glenna J Brown, Erin L Karschner et al. 4 citations
A new high-throughput urine screening and confirmation method detects psilocin, mitragynine, phencyclidine, ketamine, norketamine, and dehydronorketamine using liquid chromatography-tandem mass spectrometry. Automated liquid handling with dispersive pipette extraction tips prepares screening samples, while offline solid-phase extraction handles confirmation samples. The method achieves limits of detection between 1-5 ng/mL for screening and 1 ng/mL for confirmation, using smaller specimen volumes than previous methods. Validation followed ANSI/ASB Standard 036 for forensic toxicology. Automation improves throughput and quality assurance, making the method suitable for workplace drug testing, human performance, and postmortem laboratories needing robust analysis of these traditionally challenging analytes.