Simultaneous quantitative determination of 2-fluoro-2-oxo-phenylcyclohexylethylamine, methylenedioxymethamphetamine and ketamine in postmortem blood using liquid chromatography-tandem mass spectrometry.
Forensic toxicology January 1, 2026 Meejung Park, Sungmin Moon, Nahyun Lee et al.
A validated method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with solid-phase extraction was developed for simultaneously quantifying 2F-2-oxo-PCE, MDMA, MDA, ketamine, and norketamine in postmortem blood. The method showed satisfactory linearity, accuracy, precision, matrix effect, and recovery. In autopsy cases, 2F-2-oxo-PCE concentrations ranged from 664 to 7911 ng/mL. Concurrent detection with MDMA and ketamine suggested possible polydrug use contributing to fatal outcomes. This is the first forensic toxicological detection of 2F-2-oxo-PCE in autopsy cases, providing critical baseline data for interpreting intoxications involving this emerging dissociative substance.