Jean Claude Alvarez

Department of Pharmacology and Toxicology, Raymond Poincaré Hospital, AP-HP, Garches 92380, France.

2 papers in the library · 31 citations · publishing 2021-2023

Papers

Metabolic profiling of deschloro-N-ethyl-ketamine and identification of new target metabolites in urine and hair using human liver microsomes and high-resolution accurate mass spectrometry.

Drug testing and analysis June 1, 2021 Islam Amine Larabi, Fanny Zerizer, Alice Ameline et al. 23 citations

A new ketamine analogue, deschloro-N-ethyl-ketamine (O-PCE), involved in severe intoxications and deaths, was metabolically profiled for the first time. After incubating O-PCE with human liver microsomes and analyzing urine and hair from a 43-year-old male user using high-resolution mass spectrometry, 15 metabolites were identified. Nine metabolites detected in urine extended the detection window after O-PCE itself was no longer present. The five most abundant urinary markers were 2-en-PCA-N-Glu (34%), M3 (16%), O-PCA-N-Glu (15.4%), OH-O-PCE (15%), and OH-PCE (11.9%). In hair, nine metabolites appeared; OH-PCA dominated (78%) with a metabolite-to-parent-drug ratio of 6, making it the best marker for long-term monitoring of O-PCE exposure.

DOI: 10.1002/dta.3007 Full text Deschloro-n-ethyl-ketamine Hair High-resolution mass spectrometry Human liver microsomes +1

Characterization of extensive 2-fluorodeschloroketamine metabolism in pooled human liver microsomes, urine and hair from an addicted patient using high-resolution accurate mass spectrometry.

Journal of analytical toxicology July 22, 2023 Delphine Joseph, Camille Lesueur, Fanny Zerizer et al. 8 citations

Twenty-six potential metabolites of the ketamine derivative 2-fluorodeschloroketamine (2F-DCK) were identified, with 15 reported for the first time. Thirteen metabolites were detected in pooled human liver microsomes, 10 were confirmed in both urine and hair from a chronic user, and all were found in at least one of those samples. Nor-2F-DCK is confirmed as a reliable target analyte; OH-dihydro-nor-2F-DCK and dehydro-nor-2F-DCK are suggested as new target analytes in urine and hair, respectively. Deschloroketamine (DCK) was identified as a 2F-DCK metabolite for the first time, with concentrations in hair segments A (0–3 cm), B (3–6 cm), and C (6–9 cm) of 885, 1,500, and 1,850 pg/mg, respectively. Two seized crystals contained 2F-DCK at 67% and 96% with traces of DCK (0.4% and 0.6%) from cross-contamination.

DOI: 10.1093/jat/bkad030 Full text