Metabolite elucidation of 2-fluoro-deschloroketamine (2F-DCK) using molecular networking across three complementary in vitro and in vivo models.
Drug testing and analysis January 1, 2022 Thomas Gicquel, Romain Pelletier, Camille Richeval et al. 32 citations
Thirteen metabolites of the dissociative drug 2-fluoro-deschloroketamine (2F-DCK) were produced in vitro using human liver microsomes and HepaRG liver cells. Seven additional metabolites, including three Phase II conjugates, were identified in post-mortem bile and urine from a fatal case. Molecular networking helped compare the two in vitro models, which proved complementary. The authors propose that nor-2F-DCK (mass-to-charge 208.1137) and a hydrogenated metabolite (224.1443) are reliable markers for detecting 2F-DCK use in high-resolution mass spectrometry libraries.