125I-lysergic acid diethylamide binds to a novel serotonergic site on rat choroid plexus epithelial cells
Journal of Neuroscience December 1, 1985 K A Yagaloff, Hartig Pr 159 citations
A binding site for lysergic acid diethylamide (LSD) on the rat choroid plexus, a brain structure that produces cerebrospinal fluid, was found to be a new type of serotonergic site. Using a high-resolution autoradiography technique, the site was localized to the epithelial cells of the choroid plexus. The density of this site was 3100 fmol/mg of protein, ten times higher than any other serotonergic site in brain homogenates. The site's pharmacology was distinct from known serotonin receptor types 5-HT1a, 5-HT1b, and 5-HT2. Binding was strongly inhibited by mianserin, serotonin, and (+)-LSD, while other serotonergic, dopaminergic, and adrenergic agents had moderate to weak effects. The site appears to be located on non-neuronal cells.