In Vitro Screening of Psychoactive Drugs by [35S]GTP.GAMMA.S Binding in Rat Brain Membranes
Biological and Pharmaceutical Bulletin January 1, 2007 Ryouichi Nonaka, Fumiko Nagai, Akio Ogata et al. 31 citations
A new small-scale method measures how psychoactive drugs activate monoamine receptors by tracking G protein binding in rat brain membranes. The method simultaneously tests dopamine, serotonin, and norepinephrine effects. Among 12 phenethylamines tested, only 2C-C, 2C-E, and 2C-I stimulated G protein binding; the rest had no effect. All 7 tryptamines tested stimulated binding, with 5-MeO-DMT being most potent, followed by 5-MeO-DALT, 5-MeO-AMT, 5-MeO-MIPT, 5-MeO-DIPT, DPT, and AMT. The assay can help classify psychoactive substances as prohibited under Tokyo Metropolitan criteria.