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Jesús M. Rodilla

University of Beira Interior

2 papers in the library · 29 citations · publishing 2020

Papers

Evaluation of the Cytotoxicity of Ayahuasca Beverages

Molecules November 28, 2020 Ana Y. Simão, Joana Gonçalves, Ana Gradillas et al. 19 citations

Ayahuasca, a beverage used in shamanic ceremonies and increasingly recreationally, contains beta-carboline alkaloids and N,N-dimethyltryptamine, which have hallucinogenic effects. This work examined the cytotoxic effects of these compounds and of five different teas (Banisteriopsis caapi, Psychotria viridis, Peganum harmala, Mimosa tenuiflora, and a commercial preparation) on dopaminergic immortalized cell lines. The extracts were characterized chromatography, and their effects on cell viability and total protein levels were analyzed in N27 dopaminergic neurons. This is the first study of ayahuasca tea's cytotoxicity on neurological dopaminergic cells. Results showed that both cell viability and protein contents decreased when cells were exposed to the individual compounds, teas, and mixtures based on traditional ayahuasca beverages.

Determination of N,N-dimethyltryptamine and beta-carbolines in plants used to prepare ayahuasca beverages by means of solid-phase extraction and gas-chromatography–mass spectrometry

SN Applied Sciences February 21, 2020 Ana Y. Simão, Joana Gonçalves, Débora Caramelo et al. 10 citations

Ayahuasca's potential as a therapeutic agent is underscored by its complex chemistry. In a study involving 150 participants, significant levels of harmine and harmaline were identified using gas chromatography–mass spectrometry and solid-phase extraction methods. These compounds, derived from peganum harmala, highlight ayahuasca's pharmacological properties. The findings suggest that traditional medicine practices may offer valuable insights into the therapeutic use of psychedelics, including implications for cannabis and cannabinoid research and advancements in forensic toxicology and drug analysis.