Selina Kraus

Division of Clinical Pharmacology and Toxicology, Department of Biomedicine, University Hospital Basel, Basel, Switzerland; Division of Clinical Pharmacology and Toxicology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland.

2 papers in the library · 5 citations · publishing 2025

Papers

Liquid chromatography-tandem mass spectrometry-based pharmacokinetic and metabolic analysis of 4-bromo-2,5-dimethoxyphenethylamine and its metabolites in human plasma.

Drug metabolism and disposition: the biological fate of chemicals April 28, 2025 Jan Thomann, Deborah Rudin, Selina Kraus et al. 4 citations

A liquid chromatography-tandem mass spectrometry method was developed and validated to measure the recreational psychedelic 2C-B and two of its metabolites (BDMPAA and B-2-HMPAA) in human plasma. The method achieved linear ranges of 0.5–100 ng/mL for 2C-B, 2.5–1000 ng/mL for BDMPAA, and 0.5–1000 ng/mL for B-2-HMPAA with high accuracy and precision. Pharmacokinetic analysis used samples from clinical participants who received 30 mg of 2C-B. Key metabolic enzymes included MAO-A, MAO-B, cytosolic enzymes, and CYP2D6. Unlike 2C-B, the metabolites did not activate the serotonin 2A receptor, indicating they do not contribute to the psychedelic effect. The method provides a reliable tool for future clinical studies.

DOI: 10.1016/j.dmd.2025.100086 Full text Bioanalytical chemistry Bioanalysis Method validation Pharmacokinetics +5

Development and Validation of a Rapid LC-MS/MS Method for Plasma Analysis of Ketamine, Norketamine, Dehydronorketamine, and Hydroxynorketamine.

Biomedical chromatography : BMC September 1, 2025 Jan Thomann, Selina Kraus, Livio Erne et al. 1 citation

A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method accurately measures ketamine and its metabolites norketamine, dehydronorketamine (DHNK), and (2R,6R)-hydroxynorketamine (HNK) in human plasma. The method uses a small sample volume, a simple protein precipitation step, and a fast run time. Linear quantification ranges were 1-1,000 ng/mL for ketamine and norketamine, 0.25-100 ng/mL for DHNK, and 2.5-1,000 ng/mL for (2R,6R)-HNK. The method showed high accuracy, precision, selectivity, and sensitivity, with consistent matrix effects and efficient extraction recovery. It was successfully applied to assess pharmacokinetics in six clinical trial participants, offering a robust approach for clinical studies, drug monitoring, and forensic investigations.

DOI: 10.1002/bmc.70171 Full text Bioanalysis Clinical study Liquid chromatography Mass spectrometry +5