Screening method for the analysis of blood and urine for the presence of naturally occurring toxic compounds from mushrooms (fungi) using the LC-MS method
Problems of Forensic Sciences March 29, 2022 Bartłomiej Feigel, Ariusz Zuba, Wojciech Lechowicz 2 citations
A qualitative method using solid phase extraction and liquid chromatography with quadrupole time-of-flight mass spectrometry (LC/QTOF) was developed to detect five fungal toxins—α-amanitin, β-amanitin, γ-amanitin, muscarine, and psilocin—in blood and urine. The method uses HLB columns and acetonitrile-based extraction, with detection limits ranging from 0.3 to 2.1 ng/mL depending on the compound and matrix. It enables efficient identification of these toxins in forensic toxicology, addressing challenges in analyzing biological material from autopsies or living humans for mushroom poisoning.