New Synthesis and Characterization of (+)-Lysergic Acid Diethylamide (LSD) Derivatives and the Development of a Microparticle-Based Immunoassay for the Detection of LSD and Its Metabolites
Bioconjugate Chemistry November 1, 1997 Zhuyin Li, K. Goc-Szkutnicka, A. J. Mcnally et al. 13 citations
Three lysergic acid diethylamide (LSD) derivatives were synthesized and characterized. The most stable derivative was selected and covalently linked to polystyrene microparticles through a carrier protein. Two new LSD immunogens were synthesized, generating antibodies that recognize LSD and several major LSD metabolites. A homogeneous microparticle-based immunoassay was developed for detecting LSD in human urine with sufficient sensitivity and specificity for effective screening. The assay's performance was evaluated using precision, cross-reactivity, correlation to the Abuscreen LSD RIA and GC/MS/MS, specificity, and limit of detection.