Validation of an analytical method for the determination of the main ayahuasca active compounds and application to real ayahuasca samples from Brazil
Journal of Chromatography B June 8, 2019 Rita C. Z. Souza, Flávia Da Silva Zandonadi, Donizete P. Freitas et al. 42 citations
A fast, validated analytical method for quantifying the main active compounds in ayahuasca—N,N-dimethyltryptamine (DMT), tetrahydroharmine (THH), harmine (HME), and harmaline (HML)—was developed and applied to 38 samples from São Paulo, Brazil. The method shows minimal matrix interference and high reproducibility, enabling the tracing of active compound concentrations for the first time. This tool supports studies linking compound levels to biological responses using multi-omic platforms.