Two-Dimensional Gas Chromatography/Electron-Impact Mass Spectrometry with Cryofocusing for Simultaneous Quantification of MDMA, MDA, HMMA, HMA, and MDEA in Human Plasma
Clinical Chemistry December 19, 2007 Erin A Kolbrich, Ross H. Lowe, Marilyn A. Huestis 36 citations
A two-dimensional gas chromatography–mass spectrometry method with cryofocusing simultaneously quantifies MDMA, its metabolites MDA, HMMA, and HMA, and MDEA in human plasma. Limits of quantification were 1.0 μg/L for MDA and 2.5 μg/L for the other four compounds. Calibration curves were linear up to 100 μg/L for MDA and HMA and up to 400 μg/L for MDEA, MDMA, and HMMA, with correlation coefficients above 0.997. Extraction efficiencies from plasma were at least 85%, recoveries ranged from 85.6% to 107.2% of target, and intra- and interassay imprecision was below 8.5% at three concentrations. None of 66 tested exogenous compounds interfered. The assay achieves low quantification limits suitable for pharmacokinetic studies and may apply to other analytes and complex matrices.