A new ultra-high-performance liquid chromatography-tandem mass spectrometry method was developed to measure ayahuasca alkaloids in seized herbal products. The method reliably quantifies N,N-dimethyltryptamine (DMT), harmine, harmaline, and tetrahydroharmine, with detection limits of 10 ng/mL for DMT and 25 ng/mL for the β-carbolines. Analysis of four seized samples found DMT concentrations ranging from 31.5 to 46.5 mg/g, but no β-carbolines were detected. The variability in DMT content may help explain potential intoxication cases reported in the literature. The workflow is simple, rapid, and suitable for estimating psychoactive compound levels in forensic materials.
A simple method using protein precipitation and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed to detect 16 stimulant substances in postmortem blood samples from nearly 1000 Brazilian forensic cases. The method was validated according to ANSI/ASB Standard 036 and showed low quantification limits (5–20 ng/mL) and good precision and accuracy. Analysis of 971 samples found that about 20.1% tested positive for at least one substance, with benzoylecgonine (17.8%), ecgonine methyl ester (13.9%), and cocaine (13.0%) being most common. Significant matrix effects occurred only for EME and phenylephrine.