A method for detecting and identifying ibogaine and heroin in biological samples is described. The procedure uses thin-layer chromatography and specific color reagents to distinguish these substances from other alkaloids. The technique is intended for forensic toxicology and drug analysis applications.
A thin layer chromatography method separates and identifies four psychoactive substances—LSD, DMT, STP, and ibogaine—in biological samples and seized materials. The technique uses specific solvent systems and visualization reagents to produce distinct retention factor values for each compound, enabling forensic detection. The method reliably distinguishes these drugs from common adulterants and other alkaloids. The authors provide detailed procedures for sample preparation, plate development, and spot visualization, including the use of ultraviolet light and chemical sprays. This approach offers a practical tool for criminalistics laboratories to confirm the presence of these substances in forensic casework.