Cytotoxic, genotoxic, and oxidative stress-related effects of lysergic acid diethylamide (LSD) and phencyclidine (PCP) in the human neuroblastoma SH-SY5Y cell line
Arhiv za Higijenu Rada i Toksikologiju April 1, 2022 Jurič Andreja, Zandona Antonio, Lovaković Blanka Tariba et al. 6 citations
Lysergic acid diethylamide (LSD) and phencyclidine (PCP) both induce DNA damage and oxidative stress in human neuroblastoma SH-SY5Y cells after 24-hour exposure. LSD at 6.25 μmol/L reduced cell viability to 88.06±2.05% and PCP at 3.13 μmol/L to 84.17±3.19%, with both causing a significant increase in primary DNA damage. LSD increased malondialdehyde, reactive oxygen species (ROS), and glutathione (GSH) levels, and raised activities of all measured antioxidant enzymes. PCP increased ROS but lowered GSH, and increased superoxide dismutase and glutathione peroxidase while decreasing catalase activity. LSD appears to have greater DNA damaging potential and stronger oxidative activity than PCP in this cell line.