Determination of synthetic hallucinogens in oral fluids by microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry.
Analytical and bioanalytical chemistry July 1, 2023 Evan Lesne, Miguel Muñoz-Bartual, Francesc A Esteve-Turrillas 14 citations
A fast and simple method using microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry was developed to simultaneously quantify 28 synthetic hallucinogens in oral fluids, including LSD and substances from NBOMe, NBOH, NBF, 2C, and substituted amphetamine classes. Optimal extraction used C18 sorbent, 100 μL sample at pH 7 loaded in 3 cycles, washing with water, and eluting with 50 μL methanol in 1 cycle, yielding quantitative recoveries (80–129%) and no significant matrix effects. Limits of detection ranged from 0.09 to 1.22 μg L⁻¹. Precision was high, with relative standard deviations below 9%. The method is appropriate for simple, sensitive determination of NBOMe derivatives and other synthetic hallucinogens in oral fluid samples.