Genetische und biochemische Grundlage der Indolalkaloidbildung in Basidiomyceten und biotechnologische Produktion des Psilocybins
Common Library Network (Der Gemeinsame Bibliotheksverbund) January 1, 2020 Janis Fricke
The structural diversity and genetic and enzymatic foundations of indole alkaloid biosynthesis in Basidiomycetes of Psilocybe spp. were investigated. Through in silico analyses and heterologous expression, the biosynthesis of psilocybin was elucidated, identifying the responsible genes and biochemically and phylogenetically characterizing the resulting enzymes. Psilocybin biosynthesis is catalyzed by four enzymes: L-tryptophan decarboxylase PsiD, cytochrome P450 monooxygenase PsiH, kinase PsiK, and N-methyltransferase PsiM, which are unique secondary metabolite enzymes whose substrate specificities revealed a sequence differing from the previously postulated pathway.