Determination of MDMA and MDA in rat urine by semi‐micro column HPLC‐fluorescence detection with DBD‐F and their monitoring after MDMA administration to rat
Luminescence May 1, 2005 Mitsuhiro Wada, Shinichi Nakamura, Mamoru Tomita et al. 14 citations
A method using high-performance liquid chromatography with fluorescence detection was developed to measure the abused drugs MDMA, MDA, amphetamine, and methamphetamine in rat urine. The drugs were labeled with a fluorescent reagent and separated within 45 minutes. Detection limits ranged from 0.5 to 15 ng/mL. After a single injection of MDMA to rats, both MDMA and its metabolite MDA could be detected in urine for up to 15 hours, with concentrations ranging from 0.13 to 160.1 µg/mL for MDMA and 0.17 to 10.9 µg/mL for MDA.