LSD and 9,10‐dihydro‐LSD Analyses in Street Drug Blotter Samples via Easy Ambient Sonic‐Spray Ionization MassSpectrometry (EASI‐MS)
Wanderson Romão, Bruno Duarte Sabino, Maria Izabel M. S. Bueno, Boniek G. Vaz, Amadeu C. Júnior, Adriano O. Maldaner, Eustáquio Vinícius Ribeiro de Castro, Rogério Araújo Lordeiro, Clésia C. Nascentes, Marcos N. Eberlin, Rodinei Augusti
Journal of Forensic Sciences August 6, 2012 DOI: 10.1111/j.1556-4029.2012.02237.x via OpenAlex
Summary
AI-generated from the abstractForensic identification of LSD typically relies on the Ehrlich spot test, which is nonspecific and cannot distinguish LSD from the uncontrolled substance 9,10-dihydro-LSD, recently found in Brazilian blotters. This work used easy ambient sonic-spray ionization mass spectrometry (EASI-MS) to characterize LSD and 9,10-dihydro-LSD directly from blotter surfaces. Of 41 blotters analyzed by EASI-MS, 28 tested positive for LSD, seven for 9,10-dihydro-LSD, and six for neither. Results were confirmed by high-performance liquid chromatography. Combining thin-layer chromatography with EASI-MS proved a simple, powerful screening tool for forensic drug analysis.
Study at a glance
| Characteristics | Method development and validation study Peer reviewed |
|---|---|
| Sample size | 41 |
| Population | Seized blotter samples containing LSD or 9,10-dihydro-LSD |
| Keywords | Designer drug Chromatography Chemistry Mass spectrometry Drug detection |
| Citations | 24 |
| Key finding | EASI-MS successfully distinguished LSD from 9,10-dihydro-LSD in seized blotters, with 28 of 41 samples positive for LSD, seven for 9,10-dihydro-LSD, and six negative for both. |
Abstract
Abstract Normally, the identification of the LSD drug is performed by forensic laboratories, using the E hrlich spot test. However, this is a nonspecific analysis. Additionally, the Brazilian Federal Police has identified the presence of a new compound in seized blotters: 9,10‐dihydro‐ LSD , an uncontrolled substance. In this work, easy ambient sonic‐spray ionization mass spectrometry in the positive ion mode, EASI (+)‐ MS , was used to characterize LSD and 9,10‐dihydro‐ LSD compositions directly from the surface of blotters. The presence of LSD in the seized blotter samples were also confirmed via high‐performance liquid chromatography with ultraviolet detector. In a set of 41 blotters analyzed by EASI (+)‐ MS , 28 showed positive results for LSD , seven for 9,10‐dihydro‐ LSD , and another six samples showed negative results for both LSD and 9,10‐dihydro‐ LSD . The combination of thin layer chromatography with EASI ‐ MS also demonstrated to be a relatively simple and powerful screening tool for forensic analysis of street drugs.