Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes
Teodora Vidonja Uzelac, Nikola Tatalović, Milica Mijović, Gordana Koželj, Aleksandra Nikolić‐kokić, Zorana Oreščanin-dušić, Mara Bresjanac, Duško Blagojević
Archives of Biological Sciences December 5, 2018 DOI: 10.2298/abs180918055v via OpenAlex
Summary
AI-generated from the abstractA single oral dose of ibogaine (1 or 20 mg/kg body weight) in rats did not alter the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase, or glutathione-S-transferase in the liver or erythrocytes at 6 or 24 hours after administration. However, hepatic xanthine oxidase activity increased in rats receiving 20 mg/kg, indicating faster adenosine turnover. TBARS concentration rose in the 1 mg/kg group after 24 hours, suggesting mild oxidative stress. Histological examination revealed glycogenolytic activity in hepatocytes, peaking at 24 hours in the higher-dose group. Ibogaine influenced hepatic redox homeostasis but not enough to remodel antioxidant enzyme activities within the timeframe studied.
Study at a glance
| Characteristics | Animal study Peer reviewed |
|---|---|
| Population | Rat liver and erythrocytes |
| Intervention | Ibogaine |
| Dose | 1 or 20 mg/kg body weight |
| Duration | 6 and 24 hours post-administration |
| Keywords | Oxidative stress Pharmacology Superoxide dismutase Glutathione peroxidase Antioxidant |
| Citations | 7 |
| Key finding | Ibogaine treatment influenced hepatic redox homeostasis without altering the activities of key antioxidant enzymes in the liver or erythrocytes at 6 and 24 hours post-administration. |
Abstract
Ibogaine, administered as a single oral dose (1-25 mg/kg body weight), has been used as an addiction-interrupting agent. Its effects persist for up to 72 h. Ex vivo results showed that ibogaine induced cellular energy consumption and restitution, followed by increased reactive oxygen species production and antioxidant activity. Therefore, the aim of this work was to explore the effect of a single oral dose of ibogaine (1 or 20 mg/kg body weight) on antioxidative defenses in rat liver and erythrocytes. Six and 24 h after ibogaine administration, histological examination showed glycogenolytic activity in hepatocytes, which was highest after 24 h in animals that received 20 mg/kg ibogaine. There were no changes in the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase in the liver and erythrocytes after ibogaine treatment, regardless of the dose. Hepatic xanthine oxidase activity was elevated in rats that received 20 mg/kg compared to the controls (p<0.01), suggesting faster adenosine turnover. TBARS concentration was elevated in the group treated with 1 mg/kg after 24 h compared to the controls (p<0.01), suggesting mild oxidative stress. Our results show that ibogaine treatment influenced hepatic redox homeostasis, but not sufficiently to remodel antioxidant enzyme activities at 6 and 24 h post-ibogaine application.