Sensitive determination of alpha-methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine using gas chromatography-mass spectrometry.
Tomomi Ishida, Keiko Kudo, Akiko Kiyoshima, Hiromasa Inoue, Akiko Tsuji, Noriaki Ikeda
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences August 25, 2005 DOI: 10.1016/j.jchromb.2004.10.067 via PubMed
Summary
AI-generated from the abstractA sensitive method using gas chromatography-mass spectrometry (GC-MS) was developed to measure alpha-methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine. The drugs were extracted with an Extrelut column and an internal standard, then derivatized with acetic anhydride. Calibration curves were linear from 10 to 750 ng/ml in both blood and urine. The method detection limit was 1 ng/ml for each drug in whole blood and 5 ng/ml in urine. This approach accurately detects these drugs in clinical and forensic samples.
Study at a glance
| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Key finding | A GC-MS method was developed that can detect AMT and 5MeO-DIPT in blood and urine with detection limits of 1 ng/ml in blood and 5 ng/ml in urine. |
Abstract
We devised a sensitive and simple method to determine alpha-methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine, using gas chromatography-mass spectrometry (GC-MS). AMT and 5MeO-DIPT were extracted using an Extrelut column with an internal standard, bupivacaine, followed by derivatization with acetic anhydride. The derivatized extract was used for GC-MS analysis of EI-SIM mode. The calibration curves of AMT and 5MeO-DIPT were linear in the concentration range from 10 to 750 ng/ml in both blood and urine samples. The method detection limit (MDL) of AMT and 5MeO-DIPT were 1 ng/ml each in whole blood and 5 ng/ml each in urine. This method should be most useful to accurately determine the presence of these drugs in blood and urine in clinical and forensic cases.