Quantification of Phencyclidine (PCP) in Urine, Serum, or Plasma by Gas Chromatography-Mass Spectrometry (GC-MS).
David Scott, C Clinton Frazee, Uttam Garg
Methods in molecular biology (Clifton, N.J.) January 1, 2024 DOI: 10.1007/978-1-0716-3541-4_37 via PubMed
Summary
AI-generated from the abstractPhencyclidine (PCP), a dissociative anesthetic originally tested in the 1950s as an intravenous anesthetic, was discontinued for clinical use due to severe adverse effects. It is now a commonly abused recreational drug that induces hallucinations and alters perception. PCP can be detected in urine, serum, or plasma by immunoassays, and its presence is confirmed and quantified by gas or liquid chromatography-mass spectrometry. The described method adds a deuterated internal standard, extracts the drug under alkaline conditions, and uses gas chromatography-mass spectrometry with selected ion monitoring for quantitation.
Study at a glance
| Characteristics | Peer reviewed |
|---|---|
| Keywords | Drugs of abuse Gas chromatography Mass spectrometry Pcp Phencyclidine |
| Key finding | PCP can be detected and quantified using gas chromatography-mass spectrometry with a deuterated internal standard and alkaline extraction. |
Abstract
Phencyclidine (PCP), a dissociative anesthetic, is a commonly abused recreational drug. In the 1950s, initially tested as an intravenous anesthetic, PCP was discontinued for clinical use due to its severe adverse effects. Since then, it has gained popularity as a recreational drug due to its ability to induce hallucinations and alter perception. PCP can be detected in urine, serum, or plasma by immunoassays and quantified and its presence confirmed by gas or liquid chromatography-mass spectrometry. In the method described here, a deuterated internal standard is added to the sample and the drug is extracted under alkaline conditions. Analysis is conducted using gas chromatography-mass spectrometry (GC-MS). Selected ion monitoring is used for quantitation of PCP.