Quantification of Phencyclidine (PCP) in Urine, Serum, or Plasma by Gas Chromatography-Mass Spectrometry (GC-MS).
Methods in molecular biology (Clifton, N.J.) January 1, 2024 David Scott, C Clinton Frazee, Uttam Garg
Phencyclidine (PCP), a dissociative anesthetic originally tested in the 1950s as an intravenous anesthetic, was discontinued for clinical use due to severe adverse effects. It is now a commonly abused recreational drug that induces hallucinations and alters perception. PCP can be detected in urine, serum, or plasma by immunoassays, and its presence is confirmed and quantified by gas or liquid chromatography-mass spectrometry. The described method adds a deuterated internal standard, extracts the drug under alkaline conditions, and uses gas chromatography-mass spectrometry with selected ion monitoring for quantitation.