A draft sequence reference of the Psilocybe cubensis genome
F1000Research – April 09, 2021
Source: OpenAlex
Summary
A breakthrough in understanding psychedelics reveals the entire psilocybin synthesis cluster of *Psilocybe cubensis* resides on a single 3.2Mb contig. This advance in computational biology and genetics, achieved through whole genome sequencing, provides the complete 46.6Mb genome, assembled into 32 contigs with 97.6% completeness. Such detailed biology is crucial for Psychedelics and Drug Studies, offering unprecedented insights into chemical synthesis and alkaloids, and accelerating research into alkaloids: synthesis and pharmacology.
Abstract
We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.