Acute Blockade of the Serotonin Transporter With Low Doses of Escitalopram Does Not Alter the Behavioural Responses to Acute Psilocybin
European Journal of Neuroscience – December 01, 2025
Source: OpenAlex
Summary
Contrary to prior assumptions, the serotonin transporter (5-HTT) does not directly mediate psilocybin's immediate behavioral effects. Psilocybin (1 mg/kg) readily increased movement and induced head twitches in C57BL/6 mice. However, pre-treatment with the 5-HTT inhibitor escitalopram (2.5–5 mg/kg) did not alter these responses. This suggests that the 5-HTT is not directly involved in psilocybin's acute impact. Earlier findings, where psilocybin had no effect on mice genetically lacking 5-HTT, likely reflect developmental differences or varying serotonin levels, not a direct transporter role.
Abstract
ABSTRACT The psychedelic psilocybin has gained popularity in recent years as a therapy for treatment‐resistant depression and has been reported to reduce symptoms of depression and anxiety. Psilocybin's active metabolite, psilocin, possesses a binding affinity for serotonin receptors as well as for the serotonin transporter (5‐HTT). We recently reported that in contrast to wild‐type mice, psilocybin did not induce hyperlocomotion and head‐twitch responses in mice genetically lacking 5‐HTT, suggesting an involvement of 5‐HTT in mediating these effects. To further assess the specific role of 5‐HTT in psilocybin's acute behavioural effects, we treated C57BL/6 mice with the highly selective 5‐HTT inhibitor escitalopram (2.5–5 mg/kg, i.p.) prior to psilocybin administration (1 mg/kg, i.p.), and measured acute behavioural effects including head‐twitch response and locomotor activity. We found that acute psilocybin administration increased locomotor activity and induced head twitches, and that escitalopram did not alter these effects. Our study using low doses of escitalopram reveals no direct involvement of 5‐HTT in mediating the acute effects of psilocybin in mice, and instead suggests that developmental changes and varying serotonin levels may rather explain the absence of psilocybin's acute behavioural effects previously reported in the 5‐HTT homozygous knockout mice.