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Global increases in brain glucose metabolism following acute N,N-dimethyltryptamine and harmine administration in healthy volunteers: A randomised [¹⁸F]FDG-PET study

Klemens Egger, Robert Bozsak, Helena D. Aicher, Hasan Sari, Sandra N. Poetzsch, Axel Rominger, Chantal Martin-Soelch, John W. Smallridge, Dario Dornbierer, Boris B. Quednow, Milan Scheidegger, P. Cumming

Repository for Publications and Research Data (ETH Zurich) January 1, 2026 DOI: 10.3929/ethz-c-000801722 via OpenAlex

Summary

A psychedelic dose of DMT combined with harmine, mimicking ayahuasca, globally increased cerebral glucose metabolism by 12.5% in 14 healthy males, as measured by PET scans during peak drug effects. This increase was widespread across the cortex, particularly in higher-order brain networks, and positively correlated with harmine plasma levels but not with DMT levels or subjective intensity. The finding recapitulates a classic effect seen with psilocybin, suggesting a potential metabolic signature of the psychedelic state.

Study at a glance

Characteristics Single-blind, crossover design Randomized Peer reviewed
Sample size 14
Population Healthy males
Interventions buccal DMT + harmine placebo
Dose 90 mg DMT, 120 mg harmine
Keywords Harmine Placebo Neuroimaging Metabolite Electroencephalography
Registration NCT06252506
Key finding Global cerebral glucose metabolism increased by 12.5% under DMT+harmine versus placebo, with widespread cortical increases particularly in higher-order brain networks.

Abstract

Classical psychedelics such as N,N-dimethyltryptamine (DMT) modulate consciousness via serotonergic receptor agonism, and are increasingly investigated for their psychotherapeutic potential. When combined with the monoamine oxidase A (MAO-A) inhibitor harmine—mimicking the pharmacological profile of ayahuasca—oral DMT induces a psychedelic experience lasting 4–5 h. While some neuroimaging studies have characterized effects of DMT on functional connectivity and electroencephalography its impact on cerebral energy metabolism remains largely unexplored. We assessed the cerebral metabolic rate for glucose consumption (CMRglc) with [18F]fluorodeoxyglucose positron emission tomography ([18F]FDG-PET) and linear graphic analysis following buccal DMT + harmine (90 mg DMT, 120 mg harmine) versus placebo in a single-blind, crossover design in 14 healthy males. Scans were acquired during peak drug effects (100–170 min post-administration). Global CMRglc increased by 12.5% under DMT+harmine versus placebo (t = 2.58, p = 0.011). Vertex- and network-wise analyses revealed widespread cortical increases, particularly in higher-order brain networks. Exploratory analyses found a significant positive correlation between global CMRglc and harmine plasma levels, but not with DMT plasma levels, subjective intensity ratings. A psychedelic dose of DMT + harmine globally increased cerebral glucose metabolism, recapitulating a classic finding for psilocybin, and suggesting a potential metabolic signature of the psychedelic state. Clinical trial registry name and URL incl. registration number: Molecular Imaging Study of Harmine/DMT: a Basic Research Approach (HaD-PET) https://clinicaltrials.gov/study/NCT06252506.

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