[Determination of Three Types of New Psychoactive Tryptamines in Blood by QuEChERS Combined with UPLC-MS/MS].
W Hou, Y Y Wang, Y Zhang, L P Zhang, G B Xin, S Y Qin, J F Wang
Fa yi xue za zhi August 1, 2021 DOI: 10.12116/j.issn.1004-5619.2021.310205 via PubMed
Summary
A method combining QuEChERS sample preparation with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to rapidly screen and measure three new psychoactive tryptamines—5-MeO-DALT, 5-MeO-MiPT, and 5-MeO-DiPT—in human blood. The method showed good linear relationships for each compound within specific concentration ranges (0.5–100, 0.5–100, and 0.2–100 ng/mL, respectively), with correlation coefficients above 0.99. Detection limits ranged from 0.1 to 0.2 ng/mg. Recoveries were between 84.86% and 94.57%, and both intra-day and inter-day precisions were satisfactory. The procedure is simple, rapid, and suitable for qualitative and quantitative analysis of these tryptamines in blood, offering a reference for forensic casework.
Study at a glance
| Characteristics | Method development and validation Qualitative Peer reviewed |
|---|---|
| Key finding | The QuEChERS-UPLC-MS/MS method provides good linearity, low detection limits, and high recoveries for 5-MeO-DALT, 5-MeO-MiPT, and 5-MeO-DiPT in human blood. |
Abstract
Objective To establish a method combining QuEChERS and ultra-high liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） for rapid screening and testing of three types of new psychoactive tryptamines in human blood： 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT. Methods The effects of the type of extractant, the type and dosage of salting-out agent, and the dosage of adsorbent on the test results of the three tryptamines were investigated. Blood samples were processed by QuEChERS method and then determined by UPLC-MS/MS. Results The linear relationships of 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT in human blood were good in the range of 0.5-100, 0.5-100 and 0.2-100 ng/mL, respectively, with their coefficients higher than 0.99. The limits of detection （LODs） were 0.1-0.2 ng/mg. The recoveries ranged from 84.86% to 94.57%. Intra-day and inter-day precisions were good. Conclusion The method is simple, rapid, easy to operate and has a high recovery. It is suitable for the qualitative and quantitative study of tryptamines in blood and can provide the reference for public security organs to deal with related cases.