[Determination of Three Types of New Psychoactive Tryptamines in Blood by QuEChERS Combined with UPLC-MS/MS].
Fa yi xue za zhi August 1, 2021 W Hou, Y Y Wang, Y Zhang et al.
A method combining QuEChERS sample preparation with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to rapidly screen and measure three new psychoactive tryptamines—5-MeO-DALT, 5-MeO-MiPT, and 5-MeO-DiPT—in human blood. The method showed good linear relationships for each compound within specific concentration ranges (0.5–100, 0.5–100, and 0.2–100 ng/mL, respectively), with correlation coefficients above 0.99. Detection limits ranged from 0.1 to 0.2 ng/mg. Recoveries were between 84.86% and 94.57%, and both intra-day and inter-day precisions were satisfactory. The procedure is simple, rapid, and suitable for qualitative and quantitative analysis of these tryptamines in blood, offering a reference for forensic casework.