Y Zhang

2 papers in the library · publishing 2021-2025

Papers

Inhibition of autophagy by esketamine attenuates hypoxia/reoxygenation injury in cardiomyocytes via inhibition of Ca2+/CaMKKβ/AMPK/mTOR pathway by down-regulation of transient receptor potential vanilloid 1 expression.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society April 1, 2025 Y Zhang, Q M Lu, H C Hu et al.

Esketamine protects heart muscle cells from injury caused by a lack of oxygen followed by reoxygenation, a model of heart attack damage. The protection works by blocking a cellular cleanup process called autophagy through a specific signaling chain. Esketamine reduced cell death, lowered autophagy markers, and prevented a rise in calcium inside the cells. Increasing autophagy or calcium levels weakened esketamine's protective effect. The drug acts by inhibiting the TRPV1 calcium channel, which then suppresses the CaMKKβ/AMPK/mTOR pathway. The findings suggest esketamine could be a candidate for reducing heart damage from ischemia-reperfusion injury.

DOI: 10.26402/jpp.2025.2.05 Full text Esketamine Cardiology Autophagy Cell biology +1

[Determination of Three Types of New Psychoactive Tryptamines in Blood by QuEChERS Combined with UPLC-MS/MS].

Fa yi xue za zhi August 1, 2021 W Hou, Y Y Wang, Y Zhang et al.

A method combining QuEChERS sample preparation with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to rapidly screen and measure three new psychoactive tryptamines—5-MeO-DALT, 5-MeO-MiPT, and 5-MeO-DiPT—in human blood. The method showed good linear relationships for each compound within specific concentration ranges (0.5–100, 0.5–100, and 0.2–100 ng/mL, respectively), with correlation coefficients above 0.99. Detection limits ranged from 0.1 to 0.2 ng/mg. Recoveries were between 84.86% and 94.57%, and both intra-day and inter-day precisions were satisfactory. The procedure is simple, rapid, and suitable for qualitative and quantitative analysis of these tryptamines in blood, offering a reference for forensic casework.

DOI: 10.12116/j.issn.1004-5619.2021.310205 Full text