Journal of Analytical Toxicology
January 29, 2020
Xiuying Yan, Ping Xiang, Yunli Zhao et al.
20 citations
A method using gas chromatography high-resolution mass spectrometry was developed to detect and measure the designer hallucinogen 5-MeO-DIPT in urine. Two metabolites, 5-OH-DIPT and 5-MeO-IPT, were identified in users' urine. The method accurately quantified 5-MeO-DIPT between 2 and 300 ng/mL, with a detection limit of 1 ng/mL. In drug abusers, urine concentrations ranged from 1 to 2.8 ng/mL. Stability testing showed that 5-MeO-DIPT concentration decreased by 22.8% after 24 hours at 25°C, 33.2% after 5 days at 4°C, and 38.2% after 7 days at 4°C, but was stable for 30 days at -20°C. Freezing urine samples is recommended for accurate analysis.
Forensic science international
March 1, 2023
Jiaming Zheng, Xin Wang, Jiali Zhang et al.
1 citation
A new LC-MS/MS method was developed to simultaneously extract LSD, iso-LSD, 2-oxo-3-hydroxy-LSD, and 1-propionyl-LSD from hair samples. The method was applied to hair from 18 suspected LSD users. Segmental hair analysis showed LSD concentrations decreasing from the proximal to the distal end, while 1 P-LSD was not detected in any hair segments. The authors report concentrations of LSD and iso-LSD in human hair from 18 LSD users, but note that interpreting hair analysis results for LSD remains difficult.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
November 30, 2020
Yan Shi, Roujia Wang, Shuai Yuan et al.
A rapid UHPLC-MS/MS method was developed to screen 16 tryptamines in hair samples. Twenty milligrams of hair were pulverized and processed, achieving limits of detection from 0.1 to 20 pg/mg and quantitation from 3 to 50 pg/mg. The method was applied to 191 hair samples from suspected tryptamine users; 77 tested positive for 5-MeO-DiPT, while the other 114 showed none of the targeted tryptamines or metabolites. In 34 samples, 5-MeO-DiPT and its metabolites 5-MeO-NiPT, 5-OH-DiPT, and 4-OH-DiPT were detected together. The method demonstrated good accuracy (91-114%), precision, and recovery (85-115%), making it suitable for forensic analysis of tryptamine use.
Journal of forensic and legal medicine
May 1, 2020
Xiuying Yan, Shuai Yuan, Zhiguo Yu et al.
A dried urine spot (DUS) method using LC-MS/MS was developed to measure the tryptamine hallucinogen 5-MeO-DIPT, which is unstable in liquid urine. Ten microliters of urine were spotted on a card and extracted with methanol. The method's limit of detection was 0.1 ng/ml and lower limit of quantification was 0.2 ng/ml, with accuracy between 98.2% and 103.9% and precision between 2.7% and 8.5%. 5-MeO-DIPT was more stable in DUS than in urine stored at 25 °C. When applied to urine from known users, concentrations ranged from 0.3 to 2.3 ng/ml, lower than those measured by GC-Orbitrap-MS. The small sample volume and simplicity make this useful for drug screening.