Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
August 1, 2025
Yue Xiao, Shuai Yuan, Ruxin Luo et al.
3 citations
A high-throughput screening method using direct injection was developed to detect 311 new psychoactive substances (NPS) and their metabolites in wastewater, covering synthetic cannabinoids, cathinones, phenylethylamines, tryptamines, phencyclidines, benzodiazepines, fentanyls, and piperazines. The method runs in 16 minutes, with 95.8% of targets having a limit of detection at or below 10 ng/L and recoveries between 71.01% and 119.88%. Applied to 976 real samples from a Chinese city, it identified 32 substances, with ketamine detected most frequently. This approach offers a simple analytical tool for wastewater monitoring to support early warning and assessment of drug trends.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
November 30, 2020
Yan Shi, Roujia Wang, Shuai Yuan et al.
A rapid UHPLC-MS/MS method was developed to screen 16 tryptamines in hair samples. Twenty milligrams of hair were pulverized and processed, achieving limits of detection from 0.1 to 20 pg/mg and quantitation from 3 to 50 pg/mg. The method was applied to 191 hair samples from suspected tryptamine users; 77 tested positive for 5-MeO-DiPT, while the other 114 showed none of the targeted tryptamines or metabolites. In 34 samples, 5-MeO-DiPT and its metabolites 5-MeO-NiPT, 5-OH-DiPT, and 4-OH-DiPT were detected together. The method demonstrated good accuracy (91-114%), precision, and recovery (85-115%), making it suitable for forensic analysis of tryptamine use.
Journal of forensic and legal medicine
May 1, 2020
Xiuying Yan, Shuai Yuan, Zhiguo Yu et al.
A dried urine spot (DUS) method using LC-MS/MS was developed to measure the tryptamine hallucinogen 5-MeO-DIPT, which is unstable in liquid urine. Ten microliters of urine were spotted on a card and extracted with methanol. The method's limit of detection was 0.1 ng/ml and lower limit of quantification was 0.2 ng/ml, with accuracy between 98.2% and 103.9% and precision between 2.7% and 8.5%. 5-MeO-DIPT was more stable in DUS than in urine stored at 25 °C. When applied to urine from known users, concentrations ranged from 0.3 to 2.3 ng/ml, lower than those measured by GC-Orbitrap-MS. The small sample volume and simplicity make this useful for drug screening.